Product Overview :
Annexin V-APC/7-AAD Apoptosis Kit is used for apoptosis
detection.
Annexin A5 (or Annexin V) is a cellular protein in the annexin
group. Annexin A5 is used as a probe to detect cells that have
expressed phosphatidylserine (PS) on the cell surface, an event
found in apoptosis as well as other forms of cell death. The
annexin A5 affinity assay typically uses a conjugate of annexin V
and a fluorescent or enzymatic label, biotin or other tags, or a
radioelement, in a suitable buffer (annexin V binding to PS is Ca2+
dependent). The assay combines annexin V staining of PS membrane
events with the staining of cell nucleus with PI or AAD-7 in living
cells to distinguish from dead cells. Annexin V Apoptosis Detection
Kit is based on the observation that soon after initiating
apoptosis, cells translocate the membrane phosphatidylserine (PS)
from the inner face of the plasma membrane to the cell surface.
Once on the cell surface, PS can be easily detected by staining
with a fluorescent conjugate of Annexin V, a protein that has a
high affinity for PS. Detection can be analyzed by flow cytometry
or byfluorescence microscopy.
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